Journal: Oncogene

Article Title: Therapeutic optimization of LIPA targeting to induce endoplasmic reticulum stress and cell death in ovarian cancer

doi: 10.1038/s41388-026-03689-w

Figure Lengend Snippet: A Structures of selected ERX-41 analogs. B Heatmap depicting the antiproliferative effects of selected ERX analogs on OCa cells in vitro. C MTT assay results demonstrating the potency of ERX-208 over ERX-41 on the viability of OCa cells in vitro. D – F MTT assay results showing the impact of ERX-208 on the viability of OCa cells in vitro. G , H , Colony formation assay results illustrating the effect of ERX-208 on the clonogenic potential of OCa cells in vitro. The left panel ( G ) presents representative images of colonies formed, while the right panel ( H ) quantifies the number of colonies in control versus ERX-208-treated cells. I ERX-208 induces caspase-dependent apoptosis in treated ES2 OCa cells. Caspase 3/7 activity (fold change) was measured after treatment with ERX-208 (500 nM) alone or in combination with Q-VD-Oph (caspase inhibitor), ferrostatin-1 (ferroptosis inhibitor), or necrostatin-1 (necroptosis inhibitor). Data are represented as mean ± SEM. ns not significant; *** p < 0.001; **** p < 0.0001.

Article Snippet: Established OCa cell lines ES2, OVCAR3, OV90, SKOV3, TOV21G, TOV112D, A2780 (Supplementary Table ), were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and were maintained using ATCC recommended media.

Techniques: In Vitro, MTT Assay, Colony Assay, Control, Activity Assay

Journal: Oncogene

Article Title: Therapeutic optimization of LIPA targeting to induce endoplasmic reticulum stress and cell death in ovarian cancer

doi: 10.1038/s41388-026-03689-w

Figure Lengend Snippet: A Time-course analysis of ERX-208 (1 μM) treatment on mRNA expression of ER stress-related genes, sXBP1 and CHOP, in SKOV3, OVCAR3, ES2, and OVCAR4 cells. B RT-PCR analysis showing the temporal effects of ERX-208 (1 μM) on the expression of XBP1 (unspliced XBP1 (XBP1u) and spliced XBP1 (XBP1s)) in OCa39, OVCAR8, OVCAR3, and ES2 cells. C Western blot analysis of UPR component activation in OCa39 and OVCAR8 OCa cell lines treated with ERX-208 for the indicated time points. D Transmission electron microscopy of OVCAR8 cells illustrating the effects of vehicle and ERX-208 treatments on subcellular structures after 16 h.; yellow arrows indicate the ER. Scale bar represents 100 nm.

Article Snippet: Established OCa cell lines ES2, OVCAR3, OV90, SKOV3, TOV21G, TOV112D, A2780 (Supplementary Table ), were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and were maintained using ATCC recommended media.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Activation Assay, Transmission Assay, Electron Microscopy

Journal: Oncogene

Article Title: Therapeutic optimization of LIPA targeting to induce endoplasmic reticulum stress and cell death in ovarian cancer

doi: 10.1038/s41388-026-03689-w

Figure Lengend Snippet: A Representative IHC images of tissue sections from PDE models treated with ERX-208 (1 µM), stained for Ki67, a proliferation marker. B Quantification of Ki67 staining in PDE models. Quantitative data are presented as mean ± SEM. C , D Invasion assay results illustrating the impact of ERX-208 on OCa ascites model cells. The left panel ( C ) presents representative images of invaded cells, while the right panel ( D ) quantifies the number of invaded cells following treatment with ERX-208. E – G Effects of ERX-208 on ES2 xenograft tumor models treated with a single dose of 10 mg/kg i.p. E Tumor volume measurements of mice treated with vehicle or ERX-208. F Tumor weights at the endpoint of the study. G Quantification of the number of tumor nodules in treated and vehicle groups. Data are represented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Established OCa cell lines ES2, OVCAR3, OV90, SKOV3, TOV21G, TOV112D, A2780 (Supplementary Table ), were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and were maintained using ATCC recommended media.

Techniques: Staining, Marker, Invasion Assay